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Centrifugation and Chromatography

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Overview

Centrifugation is a method in which solid or liquid particles of different densities are separated by rotating them in the tube in a horizontal circle. Chromatography is a technique of separating the constituents of a mixture of liquids. It is also used for gases. This note contains information on chromatography, centrifuge and their application.
Centrifugation and Chromatography

Centrifugation

Centrifugation is a method in which solid or liquid particles of different densities are separated by rotating them in the tube in a horizontal circle. The heavier suspended particles move down and lighter particles remain on top. In this method, the mixture is rotated at a high speed in a test tube in a machine called centrifuge.

Chromatography

Chromatography is a technique of separating the constituents of a mixture of liquids. It is also used for gases. These substances should be such that they do not go undergo a chemical reaction with each other. The substances on which the mixtures are absorbed are called absorbents. Some of them are silica gel, filter paper, cellulose powder, alumina, etc.

Paper chromatography

Paper chromatography proceeds by a mechanism which is partly partition and partly absorption. The solute mixture, generally dissolved in water, is placed on a strip chromatographic paper and a second solvent is allowed to travel along the strip. It will extra the solute as a result distribution of the solvent between the two solvents.

Application of Chromatography

This method of separation is used to separate various chemicals in chemistry. Moreover, it is used in following sectors:

  1. To separate different colours from the mixture.
  2. To identify the medicines used by a person with blood and urine.
  3. To identify the quality of a colour.
Things to remember
  • Centrifugation is a method in which solid or liquid particles of different densities are separated by rotating them in tube in a horizontal circle.
  • Chromatography is a technique of separating the constituents of a mixture of liquids. It is also used for gases.
  • Paper chromatography proceeds by a mechanism which is partly partition a partly absorption.
  • In chromatography reacting substance cannot take part.
  • It includes every relationship which established among the people.
  • There can be more than one community in a society. Community smaller than society.
  • It is a network of social relationships which cannot see or touched.
  • common interests and common objectives are not necessary for society.
Videos for Centrifugation and Chromatography
Basics of Chromatography
Centrifugation Process
Centrifugation| Separation Methods
Thin Layer Chromatography
Questions and Answers

The advantages of chromatography are:

  • It is used to separate colored substances present in natural pigments.
  • It is used to detect and identify drugs present in the blood.

No, because they are absorbed to a same extent and can’t be separated from each other.

Chromatography is a technique that is used for separation the component of a mixture by passing it through a column or sheet of an absorbing material.
Absorbent chromatography is based on the principle that "a certain absorbent can absorb different substances to different extents” When a solution containing two or more than two dissolved substances is to be separated, it is allowed to pour into a vertical glass tube packed with finely divided absorbent like alumina. The colors of dissolved substances in a solution are separated in the form of bands.

It is the process of separation very fine suspended solid particles or semi-solid from a mixture by rotating the mixture kept in a centrifuging machine at high speed.
It is done by rotating a machine at a high speed.


s.n		 filtration chromatography
1 This method is used to remove an insoluble substance from a mixture by the use of filter paper is called filtration. Chromatography is the method of separation of colours / pigments from a solution
2 It can separate insoluble substance from the mixture. This process is used to separate colour of mixture.

The applications of chromatography are:

  • To separate different colours from the mixture.
  • To identify the medicines used by a person with blood and urine.
  • To identify the quality of a colour.

Paper chromatography proceeds by a mechanism which is partly partition aand partly absorption. The solute mixture, generally dissolved in water, is placed on a strip chromatographic paper and a second solvent is allowed to travel along the strip. It will extract the solute as a result distribution of the solvent between the two solvents.

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