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Note on Centrifugation and Chromatography

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Centrifugation

Centrifugation is a method in which solid or liquid particles of different densities are separated by rotating them in the tube in a horizontal circle. The heavier suspended particles move down and lighter particles remain on top. In this method, the mixture is rotated at a high speed in a test tube in a machine called centrifuge.

Chromatography

Chromatography is a technique of separating the constituents of a mixture of liquids. It is also used for gases. These substances should be such that they do not go undergo a chemical reaction with each other. The substances on which the mixtures are absorbed are called absorbents. Some of them are silica gel, filter paper, cellulose powder, alumina, etc.

Paper chromatography

Paper chromatography proceeds by a mechanism which is partly partition and partly absorption. The solute mixture, generally dissolved in water, is placed on a strip chromatographic paper and a second solvent is allowed to travel along the strip. It will extra the solute as a result distribution of the solvent between the two solvents.

Application of Chromatography

This method of separation is used to separate various chemicals in chemistry. Moreover, it is used in following sectors:

  1. To separate different colours from the mixture.
  2. To identify the medicines used by a person with blood and urine.
  3. To identify the quality of a colour.

  • Centrifugation is a method in which solid or liquid particles of different densities are separated by rotating them in tube in a horizontal circle.
  • Chromatography is a technique of separating the constituents of a mixture of liquids. It is also used for gases.
  • Paper chromatography proceeds by a mechanism which is partly partition a partly absorption.
  • In chromatography reacting substance cannot take part.
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Very Short Questions

The advantages of chromatography are:

  • It is used to separate colored substances present in natural pigments.
  • It is used to detect and identify drugs present in the blood.

No, because they are absorbed to a same extent and can’t be separated from each other.

Chromatography is a technique that is used for separation the component of a mixture by passing it through a column or sheet of an absorbing material.
Absorbent chromatography is based on the principle that "a certain absorbent can absorb different substances to different extents” When a solution containing two or more than two dissolved substances is to be separated, it is allowed to pour into a vertical glass tube packed with finely divided absorbent like alumina. The colors of dissolved substances in a solution are separated in the form of bands.

It is the process of separation very fine suspended solid particles or semi-solid from a mixture by rotating the mixture kept in a centrifuging machine at high speed.
It is done by rotating a machine at a high speed.


s.n
filtration chromatography
1 This method is used to remove an insoluble substance from a mixture by the use of filter paper is called filtration. Chromatography is the method of separation of colours / pigments from a solution
2 It can separate insoluble substance from the mixture. This process is used to separate colour of mixture.

The applications of chromatography are:

  • To separate different colours from the mixture.
  • To identify the medicines used by a person with blood and urine.
  • To identify the quality of a colour.

Paper chromatography proceeds by a mechanism which is partly partition aand partly absorption. The solute mixture, generally dissolved in water, is placed on a strip chromatographic paper and a second solvent is allowed to travel along the strip. It will extract the solute as a result distribution of the solvent between the two solvents.

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  • Centrifugation is defined as ______.

    method in which solid or liquid particles of different densities are separated by rotating them in tube in a horizontal circle.


    none of these


    process of separation of pure crystals from a solution


    a technique of separating the constituents of a mixture of liquids


  • Chromatography is used to separate the mixture of ______.

    solid only


    solid


    liquid


    both solid and liquid


  • The process of separation of pure crystals from a solution is called ______.

    chromatography


    crystallization


    distillation


    centrifugation


  • In which method of seperation of mixtures filter paper is not used?

    sublimation
    chromatography
    crystalization
    filtration
  • Which of them is the hardest crystal?

    saphire
    diamond
    moonstone
    ruby
  • Which one of these is not the method for separation of mixture?

    calligraphy
    paper chromatography
    centrifugation
    crystallization
  • ______________ is a method in which solid or liquid particles of different densities are separated by rotating them in the tube in a horizontal circle.

    calligraphy
    paper chromatography
    crystallization
    centrifugation
  • Which of these method uses the high speed moving electric motors?

    paper chromatography
    centrifugation
    calligraphy
    crystallization
  • Which of these method is used to separate cream from milk ?

    calligraphy
    centrifugation
    paper chromatography
    crystallization
  • Which of these methods woks on the principle of absorption or attraction of one substance in the other ?

    centrifugation
    crystallization
    chromatography
    calligraphy
  • Absorbents are:

    Substances that gets absorbed in other.
    Substances that absorbs other.
    Substances that are left after evaporation.
    Substances that are left after absorption.
  • Paper chromatography uses:

    paper that is soluble in ether
    paper that is soluble in water
    paper that is partly partitioned but non absorbent
    paper that is partly partitioned and partly absorbs
  • Crystals are:

    formation of shapes from a substance that can be used to wear ornaments
    solid without regular periodic arrangement of structural unit such as atoms ions or molecules
    solid with regular periodic arrangement of structural unit such as atoms ions or molecules
    none of these are right
  • Crystals can form:

    only regular shapes
    definite geometrical shapes
    only irregular shapes
    any of the shapes
  • Crystals formed by calcium carbonate are:

    hexagonal.
    non symmetrical because it cannot form crystals.
    alum.
    cubical.
  • The process of formation of crystals is called:

    crystallification
    crystal formation
    none of the answers are right
    crystallization
  • Which of the following is not the process of crystallization?

    preparation of solution
    Solvent evaporation
    filtration of the solution
    choice of solvent
  • A technique in which separation of vatious components is acieved by utilization of diffference in the degree to which various substances are attracted on to the surface of another substance is called

    sublimation
    crystalization
    centrifugation
    chromatography
  • Which one of the following is a technique in which a filter paper is taken as adsorbent material for analysing mixture by chromatography?

    Centrifugation
    Gas liquid partition chromatography
    Paper chromatography
    Adsorption chromatography
  • Chromatography means

    cartography
    colour writing
    colour printing
    calligraphy
  • Who discovered chromatography ?

    Louis Pasture
    John Dalton
    Mikhail Tswett
    Madam Curie
  • When was chromatography invented?

    12th century
    21st century
    19th century
    20th century
  • Which one of the following is based on the principle "A certain adsorbent can adsorb different substance to different extents"?

    Adsorption chromatography
    Paper chromatography
    Gas liquid partition chromatography
    Centrifugation
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DISCUSSIONS ABOUT THIS NOTE

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Cxfg

Crystal


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biplop swornakar

urine and blood is seprated by this process


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What type of mixtures are separated by chromatography


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Saishma

Hey! It is a technique in which a filter paper is taken as an adsorbent material for analyzing mixtures


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